Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis
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چکیده
منابع مشابه
Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods.
Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting poin...
متن کاملNearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis.
Duplex DNA fragments differing by single base substitutions can be separated by electrophoresis in denaturing gradient polyacrylamide gels, but only substitutions in a restricted part of the molecule lead to a separation (1). In an effort to circumvent this problem, we demonstrated that the melting properties and electrophoretic behavior of a 135 base pair DNA fragment containing a beta-globin ...
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Brutlag, D., Atkinson, M. R., Setlow, P. & Kornberg, A. (1969) Biochem. Biophys. Res. Commun. 37, 982-989 Joyce, C. M. & Grindley, N. D. F. (1983) Proc. Natl. Acad. Sci. U .S .A . 80, 183G1834 Joyce, C. M., Ollis, D. L., Ruch, J., Steitz, T. A,, Knigsber, W. H. & Grindley, N. D. F. (19850) in Protein Structure. Function and Design (Oxender, D. ed.), UCLA Symposia in Molecular and Cellular Biolo...
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982 BioTechniques Vol. 23, No. 6 (1997) chromosome 22q. Proc. Natl. Acad. Sci. USA 93:6297-6301. 7.Levy, J. 1994. Sequencing the yeast genome: an international achievement. Yeast 10:16891706. 8.Liu, Y.G. and R.F. Whittier. 1995. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674-681. ...
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18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.9.2699